A medical assistant is preparing the autoclave for the sterilization process. Which of the following temperatures is recommended to effectively perform sterilization?

The recommended temperature for effective sterilization in an autoclave is in the range of 121-123 degrees Celsius (250-255 degrees Fahrenheit). This temperature is critical because it is optimal for achieving the high-pressure steam conditions necessary to kill bacteria, viruses, fungi, and spores, including resistant forms such as bacterial spores. When steam under pressure reaches this temperature, it can penetrate materials more effectively, ensuring that all surfaces of instruments or materials being sterilized are adequately treated.

At this temperature, sterilization typically requires a minimum exposure time, often around 15-30 minutes, depending on the load and type of items being sterilized. This range is widely accepted in healthcare settings for standard sterilization procedures and is based on established guidelines from organizations like the Centers for Disease Control and Prevention (CDC) and the World Health Organization (WHO).

Other temperature ranges mentioned in the options are either not effective for standard sterilization processes in autoclaves or may be relevant for other methods of sterilization but not for steam sterilization. Thus, the range of 121-123 degrees Celsius is the established standard for effective sterilization.